Two Gram-positive, rod-shaped bacterial strains, H101T and H207, were isolated from deep sea water collected from South-West Indian Ocean. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains were closely related to one another (100% similarity), and had the closest relationship withMicrobacterium hominis NBRC 15708T and Microbacterium insulae KCTC 19247T (98.2\u201398.3% similarities). DNA\u2013DNA hybridization value between strains H101T and H207 was 87.2 \u00b1 3.7%, and the values between the two strains and the closely related type strains were well below 70%. The two strains also shared a number of physiological and biochemical characteristics that were distinct from the closely related species, and grew at 2\u201337 °C, pH 5\u201311 and 0\u20138% (w\/v) NaCl. Both strains contained MK-12, MK-13 and MK-11 as the detected menaquinones. The peptidoglycan was of type B1γ with an interpeptide bridge D-Glu(Hyg) → Gly2 → l-Lys. The major cellular fatty acids were anteiso-C15:0, anteiso-C17:0, and iso-C16:0. Based on the genetic and phenotypic properties, it is proposed that strains H101T and H207 be classified as representatives of a novel species of the genus Microbacterium, with the name Microbacterium marinum sp. nov. The type strain is H101T (=CGMCC 4.6941T = DSM 24947T).<\/p>\n","protected":false},"excerpt":{"rendered":"
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