\u65e5\u672c\u5217\u5cf6\u306e\u5ca9\u624b\u770c\u304b\u3089\u6c96\u7e04\u770c\u304a\u3088\u3073\u6771\u30b7\u30ca\u6d77\u306e\u6d77\u5e95\u5806\u7a4d\u7269\u30fb\u751f\u7269\u30fb\u6d77\u6d5c\u7802\u8a66\u6599\u304b\u308927\u30bb\u3067\u5206\u96e2\u57f9 \u990a\u3057\u305f\u653e\u7dda\u83cc\u5408\u8a08611 \u682a\u306e\u30b3\u30ec\u30b9\u30c6\u30ed\u30fc\u30eb\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\uff08CHE) \u751f\u7523\u83cc\u3092\u63a2\u7d22\u3057\uff0c\u5e7b0 \u682a\u306e\u967d\u6027\u682a \u3092\u5f97\uff0c\u30d2\u30c3\u30c8\u7387\uff08\u4f9b\u8a66\u83cc\u682a\u6570\u306b\u5bfe\u3059\u308b\u967d\u6027\u83cc\u6570\u306e\u5272\u5408\uff09\u306f49.1\uff05\u3067\u3042\u3063\u305f\uff0e \u6700\u9ad8\u30d2\u30c3\u30c8\u7387\u6d77\u57df\u306f\u5343 \u8449\u770c\u9928\u5c71\u6e7e\u3067743% (35\u682a\u4e2d26\u682a\uff09. \u5206\u96e2\u30b5\u30f3\u30d7\u30eb\u3067\u306f\uff0c\u6d77\u5cb8\u7802\u306e\u30d2\u30c3\u30c8\u7387\u304c67.8% (90\u682a\u4e2d61 \u682a\uff09\u3068\u6700\u9ad8\u3067\u3042\u3063\u305f\uff0e \u3053\u308c\u3089\u967d\u6027300 \u682a\u4e2d\uff0cCHE \u9ad8\u6d3b\u60273\u682a\uff08\u3044\u305a\u308c\u3082\u6d77\u5e95\u5806\u7a4d\u7269\uff09\u304c\u5f97\u3089\u308c\u305f\uff1b \u6771\u4eac\u6e7e\uff082009\u5e745 \u6708\uff0c\u6c34\u6df15m,A\u682a\u3068\u547d\u540d\uff09\uff0c\u6771\u4eac\u6e7e\uff082009\u5e7411 \u6708\uff0c\u6c34\u6df120m, B\u682a\u3068\u547d\u540d\uff09, \u6771\u30b7\u30ca\u6d77\uff082009 \u5e7410 \u6708\uff0c\u6c34\u6df1300m, C \u682a\u3068\u547d\u540d\uff09. \u3053\u308c\u30893 \u682a\u306e1\u7530rDNA \u306e\u5869\u57fa\u914d\u5217\u89e3\u6790\u304b\u3089\uff0c A,B\u682a\u306f\u3068\u3082\u306bStr\u3086tomyces rubrolavendulae\uff0c\u307e\u305fC\u682a\u306fS. glauc\u52a0\u5982r\u3068\u9ad8\u3044\u76f8\u540c\u6027\u3092\u793a\u3057\u305f\uff0e<\/p>\n
A total of 611 cholesterol esterase (CHE) producing actinomycete strains were isolated and characterized from marine bottom sediments, marine organisms and beach sands collected from bays coastal and offshore waters and beaches facing the Pacific and the East China Sea around Japan. Three hundred positive strains exhibiting CHE activity were isolated. The percentage of positive strain (hit rate) was 49.1%. Highest hit rate of 74.3%(26 positive strains out of 35 strains) was observed in Tateyama Bay in Chiba Prefecture. Among the various samples, the highest hit rate of 67.8%(61 positive strains out of 90 strains) was observed in the samples from coastal sand. Among the 300 positive strains, 3 strains which had high CHE activity were selected and used for further studies. Two strains named A and B were isolated from sediment samples from Tokyo Bay in May (5 m) and October 2009 (20 m), respectively, whereas one strain named C was isolated from the sediment sample obtained from the East China Sea (300 m). From the 16S rDNA analysis strains A and B showed high homology with Str\u3086tomyces rubrolavendulae whereas strain C was close to S. glaucin\u5982r. Among the three, strain B showed stable highest CHE activity and was selected for the purification and characterization of the enzyme. The CHE was isolated from the culture supernatant of strain B by ammonium sulfate precipitation, anion-exchange chromatography and gel filtration. The final preparation showed a single band on native electrophoresis. The molecular weight of the purified CHE was 622 kDa. The CHE exists as dimer forms composed of 22.7 kDa and 39.5 kDa in water solution. The CHE was stable up to 70\u30bb and also showed pH stability from 4 to 10. The enzyme decomposed substrates such as linoleic acid cholesterol and oleic acid cholesterol.<\/p>\n","protected":false},"excerpt":{"rendered":"
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