{"id":2542,"date":"2016-10-03T00:00:00","date_gmt":"2016-10-02T16:00:00","guid":{"rendered":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e3%81%a7%e5%9f%b9%e9%a4%8a%e3%81%97%e3%81%9f%e6%a4%8d%e7%89%a9%e7%9d%80%e7%94%9f%e6%80%a7%e7%84%a1%e7%af%80%e3%82%b5%e3%83%b3%e3%82%b4%e3%83%a2%ef%bc%8c\/"},"modified":"2016-10-03T00:00:00","modified_gmt":"2016-10-02T16:00:00","slug":"%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e3%81%a7%e5%9f%b9%e9%a4%8a%e3%81%97%e3%81%9f%e6%a4%8d%e7%89%a9%e7%9d%80%e7%94%9f%e6%80%a7%e7%84%a1%e7%af%80%e3%82%b5%e3%83%b3%e3%82%b4%e3%83%a2%ef%bc%8c","status":"publish","type":"post","link":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e3%81%a7%e5%9f%b9%e9%a4%8a%e3%81%97%e3%81%9f%e6%a4%8d%e7%89%a9%e7%9d%80%e7%94%9f%e6%80%a7%e7%84%a1%e7%af%80%e3%82%b5%e3%83%b3%e3%82%b4%e3%83%a2%ef%bc%8c\/","title":{"rendered":"\u6d77\u6d0b\u6df1\u5c64\u6c34\u3067\u57f9\u990a\u3057\u305f\u690d\u7269\u7740\u751f\u6027\u7121\u7bc0\u30b5\u30f3\u30b4\u30e2\uff0c\u30e2\u30ab\u30b5\u5c5e1\u7a2e\u306e\u6210\u9577(Growth of an Epiphytic Crustose Coralline Alga (Pneophyllum sp.) Cultured in Deep Seawater)"},"content":{"rendered":"<p>\u30e2\u30ab\u30b5\u5c5e\u306e\u5c0f\u578b\u7a2e\u306f\uff0c\u63a1\u96c6\u3084\u57f9\u990a\uff0c\u89b3\u5bdf\u304c\u5bb9\u6613\uff0c\u80de\u5b50\u7740\u751f\u304c\u8fc5\u901f\u304b\u3063\u77ed\u547d\u3067\uff0c\u7121\u7bc0\u30b5\u30f3\u30b4\u30e2\u306e \u30e2\u30c7\u30eb\u3084\u6559\u6750\u751f\u7269\u3068\u306a\u308a\u3046\u308b\uff0e \u672c\u7814\u7a76\u3067\u306f\u9928\u5c71\u7523\u30e2\u30ab\u30b5\u5c5e1 \u7a2e\u306e\u80de\u5b50\u3092Grund \u6539\u5909\u57f9\u5730\uff08\u30ea\u30f3\u6fc3 \u5ea61\/4, MGM*1\/4P)\uff0c\u8868\u5c64\u6d77\u6c34\uff08SSW, pH 8.3)\uff0c\u6c72\u307f\u7f6e\u304d\u5bcc\u5c71\u6e7e\u6df1\u5c64\u6c34\uff08DSW, pH 7.9\uff09\u304a\u3088 \u3073NaOH\u3067pH\u8abf\u7bc0\u3057\u305f\u6df1\u5c64\u6c34\uff08ADSW, pH 8.3\uff09\u3067\u57f9\u990a\u3057\u305f\uff0e MGM*1\/4P\u3067\u306f80 umol\u30fbm-2\u30fbs-i \u30675 -25C\u306e5\u6761\u4ef6\uff0c\u305d\u306e20\u30bb\u306740 \u30fb100 .imol\u30fbm-2\u30fbs-i \u3092\u52a0\u3048\uff0c\u4ed6\u306e\u57f9\u990a\u6db2\u3067\u306f15\u3067\u306820\u30bb\uff0c80 imol m-2\u30fbs-i\u3068\u3057\u305f\uff0e \u52a0\u65e5\u5f8c\u306e\u85fb\u4f53\u306f\u6982\u3057\u3066\u9ad8\u6c34\u6e29\uff0c\u9ad8\u5149\u5f37\u5ea6\u3067\u5927\u304d\u304b\u3063\u305f\uff0e MGM*1\/4P\u3067\u306f2 \u30ab\u6708\u4ee5\u5185\u306b\u67af\u6b7b\u3057\u305f\u304c\uff0c\u4ed6\u306e\u57f9\u990a\u6db2\u3067\u306f\u6210\u719f\u3057\uff0cDSW \u3068SSW 15\u30bb\u3067\u306f7\u30ab\u6708\u9593\u751f\u80b2\u3057\u305f\uff0e \u4ee5\u4e0a\uff0c \u6c72\u307f\u7f6e\u304d\u6df1\u5c64\u6c34\u306f\u3084\u3084\u9178\u6027\u3067\u3042\u308b\u304c\u672c\u7a2e\u306e\u57f9\u990a\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u304b\u3081\u305f\uff0e<\/p>\n<p>  Among crustose coralline algae, epiphytic small and thin species of Pneophyllum can be a &#39;model&#39; or an &#39;experimental alga&#39;, because of its almost year-round maturation, quick attachment of spores, ephemeral nature and ease in collection, laboratory culture and microscopic observation. In the present study, an epiphytic species of Pneophyllum was collected together with its host seagrass Zostera marina at Okinoshima, Tateyama, Chiba Prefecture and released spores were cultured in various media to find the optimum culture condition. The released spores (mixtures of carpo-spores and tetraspores) were cultured in deep seawater (DSW, pumped from a depth of 384 m in Toyama Bay and stocked in the refrigerator, pH 7.9), DSW alkalized with NaOH (ADSW, pH 83), surface seawater (SSW, pH 83) and modified Grund medium (MGM) containing a quarter concentration of phosphate (MGM*1\/4P, pH 83). Water temperature was kept at 15 and 200C under long day irradiance of 80 jimol\u30fbm-2\u30fb5-1,L:D=14:10). Only in MGM-1\/4P, in which the life cycle of Pneophyllum zostericolum had been completed in our previous study, three water temperatures 5, 10 and 250C\uff08at the above irradiance) and two light intensities 40 and 100 umol\u30fbm-2\u30fbs-&#39; (at a temperature of 20C)were added to the culture series. After 20 d, it was found that thallus was larger at higher temperatures and higher light intensities but no significant differences were found except a low value in DSW at 150C. In MGM*1\/4P, all thalli died within 2 months without reaching maturation. In SSW, DSW and ADSW, thalli matured, among which those cultured in DSW (15 and 200C) and SSW(150C)survived for seven months. Therefore, pumped and stocked DSW can be a culture media for the species in spite of slightly low pH.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30e2\u30ab\u30b5\u5c5e\u306e\u5c0f\u578b\u7a2e\u306f\uff0c\u63a1\u96c6\u3084\u57f9\u990a\uff0c\u89b3\u5bdf\u304c\u5bb9\u6613\uff0c\u80de\u5b50\u7740\u751f\u304c\u8fc5\u901f\u304b\u3063\u77ed\u547d\u3067\uff0c\u7121\u7bc0\u30b5\u30f3\u30b4\u30e2\u306e \u30e2\u30c7\u30eb\u3084\u6559\u6750\u751f\u7269\u3068\u306a\u308a\u3046 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