{"id":2538,"date":"2016-10-03T00:00:00","date_gmt":"2016-10-02T16:00:00","guid":{"rendered":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/dgge%e6%b3%95%e3%81%ab%e3%82%88%e3%82%8b%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e4%b8%ad%e3%81%ae%e5%be%ae%e7%94%9f%e7%89%a9%e7%be%a4%e9%9b%86%e7%b5%84%e6%88%90%e8%a7%a3%e6%9e%90microbial-commu\/"},"modified":"2016-10-03T00:00:00","modified_gmt":"2016-10-02T16:00:00","slug":"dgge%e6%b3%95%e3%81%ab%e3%82%88%e3%82%8b%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e4%b8%ad%e3%81%ae%e5%be%ae%e7%94%9f%e7%89%a9%e7%be%a4%e9%9b%86%e7%b5%84%e6%88%90%e8%a7%a3%e6%9e%90microbial-commu","status":"publish","type":"post","link":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/dgge%e6%b3%95%e3%81%ab%e3%82%88%e3%82%8b%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e4%b8%ad%e3%81%ae%e5%be%ae%e7%94%9f%e7%89%a9%e7%be%a4%e9%9b%86%e7%b5%84%e6%88%90%e8%a7%a3%e6%9e%90microbial-commu\/","title":{"rendered":"DGGE\u6cd5\u306b\u3088\u308b\u6d77\u6d0b\u6df1\u5c64\u6c34\u4e2d\u306e\u5fae\u751f\u7269\u7fa4\u96c6\u7d44\u6210\u89e3\u6790(Microbial Community Analysis of Deep Seawater by Denaturing Gradient Gel Electrophoresis)"},"content":{"rendered":"<p>\u81ea\u7136\u754c\u3067\u3082\u5fae\u751f\u7269\u306b\u95a2\u3059\u308b\u7814\u7a76\u304c\u5c11\u306a\u3044\u6d77\u6d0b\u6df1\u5c64\u6c34\uff08DSW\uff09\u4e2d\u306b\u306f\u5168\u304f\u672a\u77e5\u306e\uff0c\u3042\u308b\u3044\u306f\u7279\u7570 \u7684\u306a\u751f\u7406\u30fb\u751f\u5316\u5b66\u7684\u8af8\u6027\u72b6\u3092\u6709\u3059\u308b\u5fae\u751f\u7269\u306e\u5b58\u5728\u304c\u671f\u5f85\u3055\u308c\u308b\u304c\uff0c\u5fae\u751f\u7269\u306b\u95a2\u3059\u308b\u57fa\u790e\u7684\u77e5\u898b\u306f \u4e4f\u3057\u3044\u306e\u304c\u73fe\u72b6\u3067\u3042\u308b\uff0e \u305d\u3053\u3067\uff0c\u672c\u7814\u7a76\u3067\u306fDSW\u4e2d\u306b\u5b58\u5728\u3059\u308b\u5fae\u751f\u7269\u306e\u4e2d\u304b\u3089\uff0c\u4e00\u822c\u7d30\u83cc\u304a\u3088\u3073 \u771f\u83cc\u3092\u5bfe\u8c61\u306b\u3057\u3066\uff0c\u5909\u6027\u5264\u6fc3\u5ea6\u52fe\u914d\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u6cd5\uff08DGGE\u6cd5\uff09\u3092\u7528\u3044\u3066\uff0c\u5fae\u751f\u7269\u7fa4\u96c6\u7d44\u6210\u3092\u305d\u308c \u305e\u308c\u8abf\u3079\u305f\uff0e \u305d\u306e\u7d50\u679c\uff0c\u5e74\u9593\u3092\u901a\u3058\u3066\u69d8\u3005\u306a\u7fa4\u96c6\u306e\u5b58\u5728\u304c\u793a\u5506\u3055\u308c\u305f\uff0e \u307e\u305f\uff0c\u6eb6\u5b58\u614b\u6709\u6a5f\u7269\u306b\u4ed8 \u7740\u3057\u3066\u3044\u308b\u5fae\u751f\u7269\uff08A\uff09\u3068\u904a\u96e2\u3057\u3066\u5b58\u5728\u3057\u3066\u3044\u308b\u5fae\u751f\u7269\uff08F) \u306e\u7fa4\u96c6\u7d44\u6210\u306f\u7570\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b \u3063\u305f\uff0e \u3055\u3089\u306b\uff0c\u591a\u6b21\u5143\u5c3a\u5ea6\u6cd5\uff08MDS\u6cd5\uff09\u3067\u89e3\u6790\u3057\u305f\u7d50\u679c\uff0c\u4e00\u822c\u7d30\u83cc\u304a\u3088\u3073\u771f\u83cc\u3068\u3082\u306b\uff0c\u7fa4\u96c6\u7d44\u6210 \u306f\u5909\u9077\u3059\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\uff0e \u307e\u305f\uff0cDGGE\u306e\u30d0\u30f3\u30c9\u3092\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u3057\uff0c\u5fae\u751f\u7269\u3092\u540c\u5b9a\u3057\u305f\u7d50 \u679c\uff0cDSW\u4e2d\u306b\u306f\u5c5e\u30ec\u30d9\u30eb\u3067\u65b0\u898f\u3068\u601d\u308f\u308c\u308b\u7d30\u83cc\u3084\u65b0\u898f\u6027\u304c\u9ad8\u3044\u3068\u601d\u308f\u308c\u308b\u771f\u83cc\u306e\u5b58\u5728\u304c\u793a\u5506\u3055\u308c \u305f\uff0e<\/p>\n<p>  Deep seawater(DSW), about which there are few microbial reports, is of interest as excellent sources of novel microorganisms or ones with unique natures; however, only a few studies have been undertaken to investigate the microbial community in DSW In this study, we examined the community structures of bacteria and fungi in DSW samples collected from the pumping stations for DSW in Izu-Akazawa in Shizuoka Prefecture by denaturing gradient gel electrophoresis (DGGE) analyses. DGGE analyses revealed that several kinds of bacteria and fungi were present in DSW and the microbial community structures of attached microbes and free-living microbes differed. In addition, multi-dimensional scaling (1VIDS) analysis of the obtained DGGE profiles in-dicated that each community structure of bacteria and fungi changed throughout the year with the exception of a few months. Furthermore, sequence analyses of the obtained DGGE profiles to identify microorganisms indicated that bacteria belonging to new genera and novel fungi were present in DSW samples collected from the pumping stations in Izu-Akazawa.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u81ea\u7136\u754c\u3067\u3082\u5fae\u751f\u7269\u306b\u95a2\u3059\u308b\u7814\u7a76\u304c\u5c11\u306a\u3044\u6d77\u6d0b\u6df1\u5c64\u6c34\uff08DSW\uff09\u4e2d\u306b\u306f\u5168\u304f\u672a\u77e5\u306e\uff0c\u3042\u308b\u3044\u306f\u7279\u7570 \u7684\u306a\u751f\u7406\u30fb\u751f\u5316\u5b66\u7684\u8af8\u6027 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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