{"id":2504,"date":"2016-10-03T00:00:00","date_gmt":"2016-10-02T16:00:00","guid":{"rendered":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/dgge-%e6%b3%95%e3%81%ab%e3%82%88%e3%82%8b%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e4%b8%ad%e3%81%ae%e6%94%be%e7%b7%9a%e8%8f%8c%e3%81%ae%e7%be%a4%e9%9b%86%e7%b5%84%e6%88%90%e8%a7%a3%e6%9e%90commu\/"},"modified":"2016-10-03T00:00:00","modified_gmt":"2016-10-02T16:00:00","slug":"dgge-%e6%b3%95%e3%81%ab%e3%82%88%e3%82%8b%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e4%b8%ad%e3%81%ae%e6%94%be%e7%b7%9a%e8%8f%8c%e3%81%ae%e7%be%a4%e9%9b%86%e7%b5%84%e6%88%90%e8%a7%a3%e6%9e%90commu","status":"publish","type":"post","link":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/dgge-%e6%b3%95%e3%81%ab%e3%82%88%e3%82%8b%e6%b5%b7%e6%b4%8b%e6%b7%b1%e5%b1%a4%e6%b0%b4%e4%b8%ad%e3%81%ae%e6%94%be%e7%b7%9a%e8%8f%8c%e3%81%ae%e7%be%a4%e9%9b%86%e7%b5%84%e6%88%90%e8%a7%a3%e6%9e%90commu\/","title":{"rendered":"DGGE \u6cd5\u306b\u3088\u308b\u6d77\u6d0b\u6df1\u5c64\u6c34\u4e2d\u306e\u653e\u7dda\u83cc\u306e\u7fa4\u96c6\u7d44\u6210\u89e3\u6790(Community Analysis of Actinomycetes in Deep Seawater using Denaturing Gradient Gel Electrophoresis(DGGE))"},"content":{"rendered":"<p>\u4f4e\u6e29\uff0c\u9ad8\u6c34\u5727\u306e\u7279\u7570\u74b0\u5883\u4e0b\u304b\u3089\u6c72\u307f\u4e0a\u3052\u3089\u308c\u305f\u6d77\u6d0b\u6df1\u5c64\u6c34\uff08DSW\uff09\u306f\uff0c\u5fdc\u7528\u5fae\u751f\u7269\u5b66\u7684\u898b\u5730\u304b\u3089 \u3082\u69d8\u3005\u306a\u5229\u7528\u304c\u671f\u5f85\u3055\u308c\u308b\u304c\uff0c\u5fae\u751f\u7269\u306b\u95a2\u3059\u308b\u57fa\u790e\u7684\u77e5\u898b\u306f\u4e4f\u3057\u3044\u306e\u304c\u73fe\u72b6\u3067\u3042\u308b\uff0e \u305d\u3053\u3067\uff0c\u672c\u7814 \u7a76\u3067\u306fDSW \u4e2d\u306b\u5b58\u5728\u3059\u308b\u5fae\u751f\u7269\u306e\u4e2d\u304b\u3089\uff0c\u8af8\u7523\u696d\u306b\u304a\u3044\u3066\u91cd\u8981\u8996\u3055\u308c\u3066\u3044\u308b\u653e\u7dda\u83cc\u306b\u7740\u76ee\u3057\uff0c\u5909 \u6027\u5264\u6fc3\u5ea6\u52fe\u914d\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u6cd5\uff08DGGE\u6cd5\uff09\u3092\u7528\u3044\u3066\uff0c\u4f0a\u8c46\u8d64\u6ca2\u30fb\u713c\u6d25\u6c96\uff08\u99ff\u6cb3\u6e7e\uff09\u30fb\u5ba4\u6238\u6c96\u306eDSW \u3068\u8868\u9762\u6d77\u6c34\uff08SSW\uff09\u306e\u653e\u7dda\u83cc\u306e\u7fa4\u96c6\u7d44\u6210\u3092\u8abf\u3079\u305f\uff0e \u305d\u306e\u7d50\u679c\uff0cDSW \u3068ssw \u3067\u306f\uff0c\u653e\u7dda\u83cc\u306e\u7fa4\u96c6\u7d44 \u6210\u306b\u5927\u304d\u306a\u76f8\u9055\u304c\u78ba\u8a8d\u3055\u308c\u305f\uff0e \u307e\u305f\uff0c\u4f0a\u8c46\u8d64\u6ca2\u306e\u7fa4\u96c6\u7d44\u6210\u3092\u591a\u6b21\u5143\u5c3a\u5ea6\u6cd5\uff08MDS) \u3067\u89e3\u6790\u3057\u305f\u3068\u3053 \u308d\uff0cSSW\u3067\u306f\u653e\u7dda\u83cc\u306e\u7fa4\u96c6\u7d44\u6210\u306f\u5927\u304d\u304f\u5909\u5316\u3057\uff0c\u5b63\u7bc0\u3054\u3068\u30fb\uff083\u6708\u4e005\u6708\uff0c6\u6708\u4e008\u6708\uff0c9\u6708\u4e0011 \u6708\uff0c12\u6708\u4e001\u6708\uff09\u306b\u7570\u306a\u3063\u305f\u7fa4\u96c6\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u50be\u5411\u304c\u3042\u3063\u305f\u304c\uff0cDSW\u3067\u306f\u7fa4\u96c6\u7d44\u6210\u306fssw\u3068 \u6bd4\u8f03\u3057\u3066\u5909\u5316\u304c\u5c11\u306a\u304f\uff0e \u5e74\u9593\u3092\u901a\u3058\u3066\u5b89\u5b9a\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\uff0e<\/p>\n<p>  Deep seawater (DSW) pumped up from unique environments such as those with low tempera-ture and high pressure is of interest as excellent sources of beneficial microorganisms; however, only a few studies have been undertaken to investigate microbial community in DSW. In this study, we examined community structures of actinomycetes in DSW and surface seawater (SSW) samples collected from three DSW pumping stations in Japan (Izu-Akazawa and Yaizu in Shizuoka Prefecture and Muroto in Kochi Prefecture). Denaturing gradient gel electrophoresis (DGGE) analyses revealed that the actinomycete community structures were markedly different between DSW and SSW samples. In addition, multi-dimensional scaling (MDS) analysis of the obtained DGGE profiles in Izu-Akazawa indicated that the actinomycete community structures in SSW varied seasonally and tended to be uniquely different structures in each season, whereas those in DSW were stable throughout the year compared with the structures in SSW.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4f4e\u6e29\uff0c\u9ad8\u6c34\u5727\u306e\u7279\u7570\u74b0\u5883\u4e0b\u304b\u3089\u6c72\u307f\u4e0a\u3052\u3089\u308c\u305f\u6d77\u6d0b\u6df1\u5c64\u6c34\uff08DSW\uff09\u306f\uff0c\u5fdc\u7528\u5fae\u751f\u7269\u5b66\u7684\u898b\u5730\u304b\u3089 \u3082\u69d8\u3005\u306a\u5229\u7528\u304c\u671f\u5f85\u3055 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[15,14],"tags":[],"class_list":["post-2504","post","type-post","status-publish","format-standard","hentry","category-15","category-14"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"mailpoet_newsletter_max":false},"uagb_author_info":{"display_name":"administrator","author_link":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/author\/wayne\/"},"uagb_comment_info":0,"uagb_excerpt":"\u4f4e\u6e29\uff0c\u9ad8\u6c34\u5727\u306e\u7279\u7570\u74b0\u5883\u4e0b\u304b\u3089\u6c72\u307f\u4e0a\u3052\u3089\u308c\u305f\u6d77\u6d0b\u6df1\u5c64\u6c34\uff08DSW\uff09\u306f\uff0c\u5fdc\u7528\u5fae\u751f\u7269\u5b66\u7684\u898b\u5730\u304b\u3089 \u3082\u69d8\u3005\u306a\u5229\u7528\u304c\u671f\u5f85\u3055...","_links":{"self":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/posts\/2504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/comments?post=2504"}],"version-history":[{"count":0,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/posts\/2504\/revisions"}],"wp:attachment":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/media?parent=2504"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/categories?post=2504"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/tags?post=2504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}