{"id":2479,"date":"2016-10-04T00:00:00","date_gmt":"2016-10-03T16:00:00","guid":{"rendered":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/%e9%a7%bf%e6%b2%b3%e6%b9%be%e3%81%ae%e6%b0%b4%e6%b7%b1687m%e3%81%8b%e3%82%89%e6%b1%b2%e3%81%bf%e4%b8%8a%e3%81%92%e3%82%89%e3%82%8c%e3%81%9f%e6%b7%b1%e5%b1%a4%e6%b0%b4%e3%81%8b%e3%82%89%e5%8d%98\/"},"modified":"2016-10-04T00:00:00","modified_gmt":"2016-10-03T16:00:00","slug":"%e9%a7%bf%e6%b2%b3%e6%b9%be%e3%81%ae%e6%b0%b4%e6%b7%b1687m%e3%81%8b%e3%82%89%e6%b1%b2%e3%81%bf%e4%b8%8a%e3%81%92%e3%82%89%e3%82%8c%e3%81%9f%e6%b7%b1%e5%b1%a4%e6%b0%b4%e3%81%8b%e3%82%89%e5%8d%98","status":"publish","type":"post","link":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/%e9%a7%bf%e6%b2%b3%e6%b9%be%e3%81%ae%e6%b0%b4%e6%b7%b1687m%e3%81%8b%e3%82%89%e6%b1%b2%e3%81%bf%e4%b8%8a%e3%81%92%e3%82%89%e3%82%8c%e3%81%9f%e6%b7%b1%e5%b1%a4%e6%b0%b4%e3%81%8b%e3%82%89%e5%8d%98\/","title":{"rendered":"\u99ff\u6cb3\u6e7e\u306e\u6c34\u6df1687m\u304b\u3089\u6c72\u307f\u4e0a\u3052\u3089\u308c\u305f\u6df1\u5c64\u6c34\u304b\u3089\u5358\u96e2\u3055\u308c\u305f\u73ea\u85fbRhaphoneis crinigera(A diatom Rhaphneis crinigera Takano isolated from deep seawater from a depth of 687m in Suruga Bay)"},"content":{"rendered":"<p>\u99ff\u6cb3\u6e7e\u6df1\u5c64\u6c34\u53d6\u6c34\u4f9b\u7d66\u65bd\u8a2d\u3067\u306f\uff0c\u6c34\u6df1687m \u304b\u3089\u5ef6\u9577\u7d047.2 km \u306e\u30b7\u30fc\u30e0\u30ec\u30b9\u7ba1\u3092\u901a\u3057\u3066\u6df1\u5c64 \u6c34\u304c\u4f9b\u7d66\u3055\u308c\u3066\u3044\u308b\uff0e \u672c\u7814\u7a76\u3067\u306f\uff0c\u65bd\u8a2d\u306e\u53d6\u6c34\u53e3\u304b\u3089\u6c72\u307f\u4e0a\u3052\u305f\u99ff\u6cb3\u6e7e\u6df1\u5c64\u6c34\u304b\u3089\u76f4\u63a5\u63a1\u6c34\u3057\u305f\u5f8c \u76f4\u3061\u306b\u5bc6\u9589\u3057\uff0c\u7d30\u5fc3\u306e\u6ce8\u610f\u3092\u6255\u3044\u306a\u304c\u3089\u57f9\u990a\u3092\u884c\u3063\u305f\u7d50\u679c\uff0c\u3053\u308c\u307e\u3067\u56fd\u5185\u6d45\u6d77\u57df\u3067\u5831\u544a\u3055\u308c\u3066\u3044\u305f \u6d77\u7523\u6d6e\u904a\u73ea\u85fbRhaphoneis crinigera Takano \u3092\u5358\u96e2\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\uff0e \u3053\u308c\u307e\u3067\u306b\u3082\u6d77\u6d0b\u6df1\u5c64\u6c34 \u53d6\u6c34\u65bd\u8a2d\u304b\u3089\u591a\u304f\u306e\u7a2e\u306e\u73ea\u85fb\u306e\u51fa\u73fe\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u304c\uff0c\u53d6\u6c34\u5f8c\u306e\u6df7\u5165\u306e\u53ef\u80fd\u6027\u3082\u3042\u3063\u305f\uff0e\u5f93\u3063\u3066\uff0c \u4eca\u56de\u306e\u5831\u544a\u306f\uff0c\u6df1\u5c64\u6c34\u7531\u6765\u3067\u3042\u308b\u3053\u3068\u304c&lsquo;\u6700\u3082\u78ba\u5b9f\u3001\u3067\uff0cR. crinigeta \u306e\u6700\u6df1\u63a1\u96c6\u8a18\u9332\u3067\u3042\u308b\u3060\u3051 \u3067\u306a\u304f\uff0c\u6df1\u5c64\u6c34\u53d6\u6c34\u7ba1\u3092\u901a\u3057\u3066\u63a1\u96c6\u3055\u308c\u305f\u3059\u3079\u3066\u306e\u73ea\u85fb\u306e\u6700\u6df1\u63a1\u96c6\u8a18\u9332\u3067\u3042\u308b\uff0e \u672c\u7a2e\u306f\u308d\u904e\u6ec5\u83cc\u3057 \u305f\u99ff\u6cb3\u6e7e\u6df1\u5c64\u6c34\u306e\u307f\u30677 \u5e74\u4ee5\u4e0a\u7d99\u4ee3\u57f9\u990a\u304c\u53ef\u80fd\u3067\u3042\u3063\u305f\uff0e<\/p>\n<p>  At some domestic deep seawater (DSW) utilization facilities, occurrence of diatom has been reported from aquaria or tap DSW. As previous studies were aimed to make in si\u98e2 diatom flora in the facilities as databases for further use, little efforts have been made to separate species contaminated after being pumped on land. In the present study, we carefully sampled a bottle of DSW pumped from Suruga Bay, central Pacific coast of Honshu, Japan, and isolated one diatom species of highly possible DSW origin. The DSW sample (700 ml) was immediately packed after tapped through a 7,200 m long seamless pipeline deployed from a depth of 687 m off Yaizu City. After culturing at a condition (20C, 25 1umol\/m2\/sec, 12L:12D) for four months (three month in still water and one month on agar plate) using filtered and sterilized DSW, a diatom was isolated among the colony of microorganisms. The diatom was identified as a planktonic pennate species, Rhaphoneis crinigera Takano by mor-phological observation using the light and scanning and transmission electron microscops. This is not only the deepest record of R. crinigera but that among all of the diatoms collected through pipelines. Surprisingly, the diatom could be passaged 79 times during 7 years by changing with filtered and sterilized DSW once a month.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u99ff\u6cb3\u6e7e\u6df1\u5c64\u6c34\u53d6\u6c34\u4f9b\u7d66\u65bd\u8a2d\u3067\u306f\uff0c\u6c34\u6df1687m \u304b\u3089\u5ef6\u9577\u7d047.2 km \u306e\u30b7\u30fc\u30e0\u30ec\u30b9\u7ba1\u3092\u901a\u3057\u3066\u6df1\u5c64 \u6c34\u304c\u4f9b\u7d66\u3055\u308c\u3066 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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