{"id":2476,"date":"2016-10-04T00:00:00","date_gmt":"2016-10-03T16:00:00","guid":{"rendered":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/%e6%b7%b1%e6%b5%b7%e5%a0%86%e7%a9%8d%e7%89%a9%e3%81%8b%e3%82%89%e5%88%86%e9%9b%a2%e3%81%97%e3%81%9f%e6%94%be%e7%b7%9a%e8%8f%8c%e3%81%ae%e7%94%9f%e7%94%a3%e3%81%99%e3%82%8b%e3%82%b1%e3%83%a9%e3%83%81\/"},"modified":"2016-10-04T00:00:00","modified_gmt":"2016-10-03T16:00:00","slug":"%e6%b7%b1%e6%b5%b7%e5%a0%86%e7%a9%8d%e7%89%a9%e3%81%8b%e3%82%89%e5%88%86%e9%9b%a2%e3%81%97%e3%81%9f%e6%94%be%e7%b7%9a%e8%8f%8c%e3%81%ae%e7%94%9f%e7%94%a3%e3%81%99%e3%82%8b%e3%82%b1%e3%83%a9%e3%83%81","status":"publish","type":"post","link":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/%e6%b7%b1%e6%b5%b7%e5%a0%86%e7%a9%8d%e7%89%a9%e3%81%8b%e3%82%89%e5%88%86%e9%9b%a2%e3%81%97%e3%81%9f%e6%94%be%e7%b7%9a%e8%8f%8c%e3%81%ae%e7%94%9f%e7%94%a3%e3%81%99%e3%82%8b%e3%82%b1%e3%83%a9%e3%83%81\/","title":{"rendered":"\u6df1\u6d77\u5806\u7a4d\u7269\u304b\u3089\u5206\u96e2\u3057\u305f\u653e\u7dda\u83cc\u306e\u751f\u7523\u3059\u308b\u30b1\u30e9\u30c1\u30ca\u30fc\u30bc\u3068\u96e3\u5206\u89e3\u6027\u30b1\u30e9\u30c1\u30f3\u306e\u5206\u89e3(Production of keratinase by an actinomycete strain isolated from deep-sea sediment and its decomposition capability on native keratins)"},"content":{"rendered":"<p>\u9e7f\u5150\u5cf6\u6e7e\u6c34\u6df1218m \u306e\u6d77\u5e95\u5806\u7a4d\u7269\u304b\u3089\u5206\u96e2\u3055\u308c\u305f\u653e\u7dda\u83cc\u306e\u30b1\u30e9\u30c1\u30ca\u30fc\u30bc\u751f\u7523\u306e\u57f9\u990a\u6761\u4ef6\u3092\u691c\u8a0e \u3057\uff0c\u3055\u3089\u306b\u57f9\u990a\u6db2\u304b\u3089\u672c\u9175\u7d20\u3092\u5358\u96e2\u30fb\u7cbe\u88fd\u3057\u3066\u8af8\u6027\u72b6\u3092\u660e\u3089\u304b\u306b\u3057\u305f\uff0e \u9ad8\u3044\u9175\u7d20\u6d3b\u6027\u306f1\/4 \u5f37\u5ea6 (25%, v\u30cev) \u306e\u4eba\u5de5\u6d77\u6c34\u6fc3\u5ea6\u3067\u898b\u3089\u308c\u305f\uff0e \u57f9\u990a\u6db2\u4e0a\u6e05\u304b\u3089\u30a4\u30aa\u30f3\u4ea4\u63db\u53ca\u3073\u30b2\u30eb\u6f01\u904e\u30af\u30ed\u30de\u30c8\u30b0\u30e9 \u30d5\u30a3\u30fc\u306b\u3088\u308a\u30b1\u30e9\u30c1\u30ca\u30fc\u30bc\u3092\u7cbe\u88fd\u3057\u3066\u5206\u5b50\u91cf\u304c\u7d043 \u4e07\u3067\uff0c\u71b1\u53ca\u3073pH \u5b89\u5b9a\u6027\u306e\u9ad8\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3057\u305f\uff0e \u307e\u305f\uff0c\u672c\u7c97\u9175\u7d20\u306f\u30ab\u30a4\u30e1\u30f3\uff0c\u30de\u30c0\u30a4\u306e\u30a6\u30ed\u30b3\uff0c\u7fbd\u6bdb\u304a\u3088\u3073\u7f8a\u6bdb\u306a\u3069\u306e\u96e3\u5206\u89e3\u6027\u30b1\u30e9\u30c1\u30f3\u3092\u539f\u578b\u3092\u7559 \u3081\u306a\u3044\u307b\u3069\u5206\u89e3\u3057\u305f\u304c\uff0c\u30a2\u30aa\u30a6\u30df\u30ac\u30e1\u306e\u7532\u7f85\u304a\u3088\u3073\u30df\u30f3\u30af\u30af\u30b8\u30e9\u306e\u30d2\u30b2\u306a\u3069\u306e\u96e3\u5206\u89e3\u6027\u30b1\u30e9\u30c1\u30f3\u306f \u307b\u3068\u3093\u3069\u5206\u89e3\u3057\u306a\u304b\u3063\u305f\uff0e<\/p>\n<p>  Cultivation conditions for keratinase production by an actinomycete strain isolated from deep-sea sediments of Kagoshima Bay at a depth of 218 m were investigated and character-ized. Maximum production of the enzyme was obtained in a quarter strength artificial seawater. Purified enzyme obtained from the culture supernatant of the strain using cation exchange chromatography and gel filtration showed an approximate molecular weight of 30,000D with thermostable and pH stable characteristics. Various native keratins such as sea sponge, scales of red seabream, feather and sheep wool were completely decomposed by the partially purified enzyme, whereas shell of green turtle and barbell of minke whale were hardly decomposed.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9e7f\u5150\u5cf6\u6e7e\u6c34\u6df1218m \u306e\u6d77\u5e95\u5806\u7a4d\u7269\u304b\u3089\u5206\u96e2\u3055\u308c\u305f\u653e\u7dda\u83cc\u306e\u30b1\u30e9\u30c1\u30ca\u30fc\u30bc\u751f\u7523\u306e\u57f9\u990a\u6761\u4ef6\u3092\u691c\u8a0e \u3057\uff0c\u3055\u3089\u306b\u57f9\u990a\u6db2\u304b\u3089 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