1.\u76ee\u7684<\/p>\n
\u8fd1\u5e74\u30d2\u30c8\u306e\u5065\u5eb7\u306b\u5fae\u751f\u7269\u304c\u6df1\u304f\u95a2\u308f\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u3064\u3064\u3042\u308b1)\uff0e\u305d\u3053\u3067\u6211\u3005\u306f\u30d2\u30c8\u3092\u69cb\u6210\u3059\u308b\u6700\u5c0f\u5358\u4f4d\u3067\u3042\u308b\u7d30\u80de\u3092\u7528\u3044\u3066, \u3053\u308c\u3092\u6d3b\u6027\u5316\u3059\u308b\u5fae\u751f\u7269\u306e\u63a2\u7d22\u3092\u9032\u3081\u3066\u6765\u305f.\u5fae\u751f\u7269\u306e\u5206\u96e2\u6e90\u3068\u3057\u3066\u306f, \u65e2\u306b\u30e6\u30cb\u30fc\u30af\u306a\u5fae\u751f\u7269\u7fa4\u96c6\u7d44\u6210\u306e\u5b58\u5728\u304c\u793a\u5506\u3055\u308c\u3066\u3044\u308b\u6d77\u6d0b\u6df1\u5c64\u6c34\uff08\u4ee5\u5f8c, DSW\uff09\u3092\u5bfe\u8c61\u306b\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u7d50\u679c, \u4f0a\u8c46\u8d64\u6ca2DSW \u3088\u308a\u5206\u96e2\u3057\u305f\u6d77\u6d0b\u7d30\u83ccVibrio gallaecicus No. 265 \u682a\uff08\u4ee5\u5f8c, No. 265<\/p>\n
\u682a\uff09\u306b\u6b63\u5e38\u30d2\u30c8\u76ae\u819a\u7dda\u7dad\u82bd\u7d30\u80de\uff08\u4ee5\u5f8c, NB1 \u7d30\u80de\uff09\u306b\u5bfe\u3059\u308b\u7d30\u80de\u8ce6\u6d3b\u52b9\u679c\u3092\u898b\u51fa\u3057\u305f. \u305d\u3053\u3067\u672c\u7814\u7a76\u3067\u306f\uff0c\u3053\u306eNo. 265 \u682a\u304c\u751f\u7523\u3059\u308b\u7d30\u80de\u8ce6\u6d3b\u56e0\u5b50\u3092\u89e3\u660e\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u305f.<\/p>\n
2.\u65b9\u6cd5<\/p>\n
\u30b0\u30eb\u30b3\u30fc\u30b9\u30fb\u30d0\u30af\u30c8\u30da\u30d7\u30c8\u30f3\u57f9\u5730\u3067No. 265\u682a\u309220\u2103\u306720 \u6642\u9593\u56de\u8ee2\u632f\u3068\u3046\u57f9\u990a\u3057, \u9060\u5fc3\u5206\u96e2\uff0816,770×g, 4\u2103, 10 \u5206\u9593\uff09\u3057\u3066\u5f97\u3089\u308c\u305f\u4e0a\u6e05\u306b\u98fd\u548c\u6fc3\u5ea6\u306e90%\u306b\u306a\u308b\u3088\u3046\u306b\u786b\u9178\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0\u3092\u6dfb\u52a0\u5f8c, 24 \u6642\u9593\u64b9\u62cc\u3057\u3066\u4e0a\u6e05\u4e2d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5869\u6790\u3057\u305f. \u6b21\u306b\u3053\u308c\u3092\u9060\u5fc3\u5206\u96e2\uff0810,730×g, 4\u2103, 40 \u5206\u9593\uff09\u3057, \u5f97\u3089\u308c\u305f\u6c88\u6bbf\u7269\u309210mM \u30c8\u30ea\u30b9\u2010\u5869\u9178\u30d0\u30c3\u30d5\u30a1\u30fc(pH 7.0)\u306b\u6eb6\u89e3\u5f8c, \u900f\u6790\u819c\uff08UC 36-32-100, \u30a8\u30fc\u30c7\u30a3\u30a2\uff09\u306b\u79fb\u3057\u306624 \u6642\u9593\u900f\u6790\u3092\u884c\u3063\u305f. \u3053\u306e\u900f\u6790\u5185\u6db2\u3092\u3055\u3089\u306b\u9650\u5916\u6ffe\u904e\u3057\uff08\u5206\u753b\u5206\u5b50\u91cf3kDa\uff09,\u5f97\u3089\u308c\u305f\u5404\u5206\u753b\u7269\u306b\u3064\u3044\u3066NB1 \u7d30\u80de\u306b\u3088\u308b\u7d30\u80de\u8ce6\u6d3b\u52b9\u679c\u306e\u8ffd\u8de1\u3092\u884c\u3063\u305f. \u7d30\u80de\u8ce6\u6d3b\u52b9\u679c\u304c\u898b\u3089\u308c\u305f\u753b\u5206\u306fSDS-PAGE \u306b\u3088\u308b\u5206\u5b50\u91cf\u63a8\u5b9a\u3092\u884c\u3046\u3068\u3068\u3082\u306b, Human bFGF ELISA \u30ad\u30c3\u30c8\uff08\u30ec\u30a4\u30d0\u30a4\u30aa\u30c6\u30c3\u30af\uff09\u306b\u4f9b\u3057\u3066bFGF \u3068\u306e\u76f8\u95a2\u6027\u3092\u8abf\u3079\u305f.<\/p>\n
3.\u7d50\u679c\u304a\u3088\u3073\u8003\u5bdf<\/p>\n
\u672c\u83cc\u306e\u57f9\u990a\u4e0a\u6e05\u3092\u786b\u5b89\u5869\u6790\u3057, \u900f\u6790\u3057\u305f\u5185\u6db2\u3092\u9650\u5916\u6ffe\u904e\u51e6\u7406\u3057\u305f\u7d50\u679c, 3kDa \u4ee5\u4e0a\u306e\u753b\u5206\u306b\u7d30\u80de\u8ce6\u6d3b\u52b9\u679c\u304c\u8a8d\u3081\u3089\u308c\u305f\u3053\u3068\u304b\u3089, \u7d30\u80de\u8ce6\u6d3b\u56e0\u5b50\u306e\u5206\u5b50\u91cf\u306f3kDa \u4ee5\u4e0a\u3067\u3042\u308b\u3068\u63a8\u5b9a\u3055\u308c\u305f. \u305d\u3053\u3067\u3053\u306e\u56e0\u5b50\u304cNB1 \u7d30\u80de\u306e\u5897\u6b96\u56e0\u5b50\u3068\u3057\u3066\u5468\u77e5\u306ebFGF \u306e\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u305f\u305f\u3081,SDS-PAGE \u304a\u3088\u3073ELISA \u6cd5\u306b\u3088\u308a\u76f8\u95a2\u6027\u3092\u8abf\u3079\u305f. \u305d\u306e\u7d50\u679c, SDS-PAGE \u3067\u306f, \u7d043.5kDa,8.5kDa \u304a\u3088\u307338kDa \u306b\u305d\u308c\u305e\u308c\u30d0\u30f3\u30c9\u304c\u51fa\u73fe\u3057, bFGF \u306e\u5206\u5b50\u91cf\u3067\u3042\u308b14-18kDa \u4ed8\u8fd1\u306b\u306f\u30d0\u30f3\u30c9\u306f\u898b\u3089\u308c\u306a\u304b\u3063\u305f. \u3055\u3089\u306bELISA \u6cd5\u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u30ec\u30d9\u30eb\u306e\u691c\u8a0e\u306b\u304a\u3044\u3066\u3082bFGF \u3068\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u3092\u793a\u3055\u306a\u3044\u3053\u3068\u304c\u308f\u304b\u3063\u305f. \u4ee5\u4e0a\u306e\u7d50\u679c\u304b\u3089No. 265 \u682a\u304c\u751f\u7523\u3059\u308b\u7d30\u80de\u8ce6\u6d3b\u56e0\u5b50\u306fbFGF \u3067\u306f\u306a\u3044\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f. \u3057\u304b\u3057,\u5fae\u751f\u7269\u304c\u751f\u7523\u3059\u308b\u7d30\u80de\u8ce6\u6d3b\u56e0\u5b50\u306b\u95a2\u3059\u308b\u5831\u544a2)\u306f\u6975\u3081\u3066\u5c11\u306a\u304f, \u672c\u7269\u8cea\u304c\u65b0\u898f\u7269\u8cea\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u671f\u5f85\u3055\u308c\u308b\u305f\u3081, \u4eca\u5f8c\u3055\u3089\u306bNo. 265<\/p>\n
\u682a\u304c\u751f\u7523\u3059\u308b\u7d30\u80de\u8ce6\u6d3b\u56e0\u5b50\u306e\u5358\u96e2\u7cbe\u88fd\u3092\u7d9a\u884c\u3057, \u57f9\u990a\u7d30\u80de\u3078\u306e\u5229\u7528\u3092\u8996\u91ce\u306b\u5165\u308c\u305f\u8a73\u7d30\u306a\u7814\u7a76\u3092\u884c\u3044\u305f\u3044\u3068\u8003\u3048\u3066\u3044\u308b.<\/p>\n","protected":false},"excerpt":{"rendered":"
1.\u76ee\u7684 \u8fd1\u5e74\u30d2\u30c8\u306e\u5065\u5eb7\u306b\u5fae\u751f\u7269\u304c\u6df1\u304f\u95a2\u308f\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u3064\u3064\u3042\u308b1)\uff0e\u305d\u3053\u3067\u6211\u3005\u306f\u30d2\u30c8\u3092\u69cb\u6210\u3059\u308b\u6700\u5c0f\u5358\u4f4d […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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\u8fd1\u5e74\u30d2\u30c8\u306e\u5065\u5eb7\u306b\u5fae\u751f\u7269\u304c\u6df1\u304f\u95a2\u308f\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u3064\u3064\u3042\u308b1)\uff0e\u305d\u3053\u3067\u6211\u3005\u306f\u30d2\u30c8\u3092\u69cb\u6210\u3059\u308b\u6700\u5c0f\u5358\u4f4d...","_links":{"self":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/posts\/2214","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/comments?post=2214"}],"version-history":[{"count":0,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/posts\/2214\/revisions"}],"wp:attachment":[{"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/media?parent=2214"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/categories?post=2214"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/etdicwp2025.etdic.org.tw\/etdic\/wp-json\/wp\/v2\/tags?post=2214"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}