Phenotypic Characterization of a Microbe Producing Substances for Oxidative Stress Resistance Isolated from the Deep Seawater in Izu-Akazawa, Japan

Abstract

In this study, we tried to isolate microbes from a bag lter (BF) which was used to remove

suspended solids from deep seawater (DSW) at a DSW pumping station in Izu-Akazawa, Shizuoka

prefecture, Japan. As a result, 941 strains were isolated and only 10 strains from all the isolates

showed a resistant e ect against oxidative stress. The one strain which showed the highest ability

of oxidative stress resistance among 10 strains was called strain “No. 586”, and its 16S rRNA

sequence analysis was performed. As a result, the homology of strain No. 586 matched

Pseudoalteromonas denitri cans JCM21248 at 98.07%. After comparing the phenotypic characteristics

of strain No. 586 and the type strain, it was shown that there were di erences in the growth

temperature and the color of the colony between the two strains. Furthermore, the resistant e ect

of strain No. 586 against oxidative stress was remarkably higher than that of the type strain.

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